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Additionally, she must reduce her intake of sugar, caffeine, vegetable oil, hydrogenated oil, alcohol, & prescription/OTC medications, which all have powerful estrogenic effects

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Indeed, when GSAAT is reduced with gradually increasing amounts of DTT prior to incubation with NEM, either one Cys (Band 1, with 0.1 mM DTT) or no Cys residue of GSAAT is accessible for mPEG binding (with DTT 1 mM) ( Figure 2C )